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bsa pbst p21  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology bsa pbst p21
    Bsa Pbst P21, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 5649 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+pbst+p21/p21/pm35120600-510-54-57
    Average 96 stars, based on 5649 article reviews
    bsa pbst p21 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Inflammatory fibroblasts mediate resistance to neoadjuvant therapy in rectal cancer.
    Article Snippet: IL-1ra protein level in rectal cancer patients’ organoids was also determined by ELISA kit (R&D System).

    Incubation:

    Article Title: Inflammatory fibroblasts mediate resistance to neoadjuvant therapy in rectal cancer.
    Article Snippet: Samples were homogenized using the Precellys 24 Homogenizer (Bertin Technologies). .. Collected lysates were mixed with 5% b-mercaptoethanol Laemmli buffer and denatured for 5 min at 95 C. Protein lysates were separated by SDS-PAGE, transferred to polyvinylidene difluoride (PVDF) membranes, blocked for 40 min in 3% milk-PBST (1% Tween 20- PBS) and incubated overnight at 4 C with the following primary antibodies diluted in 3% BSA-PBST: p21 (1:500; Santa Cruz; #471), CDK4 (1:500), cyclin D2 (1:500), CDC2/CDK1 (1:1000), cyclin A (1:500) and p16INK4a (1:1000). .. IL-1a protein levels in rectal cancer patients’ organoids, sgctrl and sgIl1a mouse organoids were determined by ELISA Kits (R&D Systems).



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